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GrainGenes Probe Report: WMC386

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC386  [ Marker Report ]
Locus	Xwmc386-5B wmc386
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10f9 Donor: Jacquemin, Gembloux Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 130 bases located on 7D, 140 bases located on 7A, 176 bases located on 7B No trimming done to sequence at Agrogene, ends not located. (GT)22 102 to 145.
Type	PCR SSR
PCR primers	Forward ATcAcTgAAAcgAAATgAgcgg at 16 length 22 bases, Tm 65 Reverse TggTTggcggTTTTTcTcTAcA at 159 length 22 bases, Tm 65
SSR size	165 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Sequence	WMC386
Source Species	Triticum aestivum