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GrainGenes Probe Report: WMC385

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC385  [ Marker Report ]
Locus	Xwmc385
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10d9.2 Donor: Jacquemin, Gembloux Apparently multiple inserts in this clone, this is the second insert. Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)26 127 to 178.
Type	PCR SSR
PCR primers	Forward gAAATggcAATggAcTcAc at 73 length 19 bases, Tm 59 Reverse ccATcATATcccTAgcAcAA at 200 length 20 bases, Tm 58
SSR size	146 bases expected, Chinese Spring
Amplification Conditions	51C anneal, complex product.
Sequence	WMC385
Source Species	Triticum aestivum