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GrainGenes Probe Report: WMC384

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC384
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10d9.1 Donor: Jacquemin, Gembloux Apparently multple inserts in this clone, this is the first insert. 5' end not located, trimmed to insert at 3' end. Bases 21 to 36 show homology to 10e8 bases 16 to 31 (16 in longest run, maxScore = 32). (CA)12 116 to 139, (CA)15 151 to 180.
Type	PCR SSR
PCR primers	Forward TggATAgTTTAccAccAAAgccA at 60 length 23 bases, Tm 65 Reverse AcATTccTgcATTcATgcTcAc at 197 length 22 bases, Tm 64
SSR size	159 bases expected, Chinese Spring
Amplification Conditions	61C anneal, complex product.
Sequence	WMC384
Source Species	Triticum aestivum