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GrainGenes Probe Report: WMC381

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC381
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10f7.1 Donor: Jacquemin, Gembloux Trimmed to insert at both ends. (CA)8 409 to 424, (GA)28 425 to 480, (GT)17 69 to 102. There are two microsatellites, the primers here are for the first.
Type	PCR SSR
PCR primers	Forward ATTAgggTTTTcgAcATgccAg at 17 length 22 bases, Tm 64 Reverse gTTgTAgcAccggATTcgAcTT at 127 length 22 bases, Tm 64
SSR size	132 bases expected, Chinese Spring
Amplification Conditions	51C anneal, complex product with many bands.
Sequence	WMC381
Source Species	Triticum aestivum