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GrainGenes Probe Report: WMC380

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC380
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10c7 Donor: Jacquemin, Gembloux No trimming done to sequence at Agrogene, ends not located. Bases 134 to 169 (complement) show homology to 5g2 bases 88 to 123 (36 in longest run, maxScore = 73). (CT)6 79 to 90, (CA)10 91 to 110.
Type	PCR SSR
PCR primers	Forward cTgAAAgATgTATTTAcgAgcA at 32 length 22 bases, Tm 57 Reverse AATTAAAAAcTgTcAcgAccAA at 167 length 22 bases, Tm 58
SSR size	157 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic (but complex) on acrylamide.;
Sequence	WMC380
Source Species	Triticum aestivum