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GrainGenes Probe Report: WMC378

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC378
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12g12.1 Donor: Mohler, Munich Multiple inserts in this clone. This is the first insert. Trimmed to insert at 5' end, extraneous linker from Munich reoved at 3' end. Bases 243 to 273 (complement) show homology to 3d2 bases 162 to 192 (31 in longest run, maxScore = 23). (CT)11 252 to 273, (CT)8 286 to 301.
Type	PCR SSR
PCR primers	Forward TTgAAgAgcTgATTgAgTAg at 146 length 20 bases, Tm 52 Reverse cgATAgAAcTgAAgATTgTc at 302 length 20 bases, Tm 52
SSR size	176 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic (but complex) on acrylamide.
Sequence	WMC378
Source Species	Triticum aestivum