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GrainGenes Probe Report: WMC377

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC377
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12d12 Donor: Mohler, Munich Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 404 to 482 (complement) show homology to 13b6 bases 47 to 125 (79 in longest run, maxScore = 89). (CA)8 186 to 201, (TC)8 213 to 228, (CA)5 230 to 239, (CA)7 242 to 255, (CA)8 296 to 311, (CT)6 335 to 346. Bases 75 to 93 show homology to 20e11 bases 140 to 158 (complement) (19 in longest run, maxScore = 46).
Type	PCR SSR
PCR primers	Forward AAATTAcATccAccAcAgAAgg at 156 length 22 bases, Tm 60 Reverse gATgAAgggTggTAggAAcAAA at 410 length 22 bases, Tm 62
SSR size	276 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear.
Sequence	WMC377
Source Species	Triticum aestivum