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GrainGenes Probe Report: WMC376

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC376  [ Marker Report ]
Locus	Xwmc376-5B Xwmc376 wmc376 wmc376-5B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12a12.2 Donor: Mohler, Munich Multiple inserts in this clone. This is the second insert. Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 65 to 115 (complement) show homology to 11b2 bases 64 to 114 (51 in longest run, maxScore = 69). (GT)16 115 to 146, (GT)17 201 to 234.
Type	PCR SSR
PCR primers	Forward TcTcAAccAccgAcTTgTAA at 33 length 20 bases, Tm 59 Reverse AcATgTAATTggggAcAcTg at 235 length 20 bases, Tm 58
SSR size	222 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide.
Sequence	WMC376
Source Species	Triticum aestivum