Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC375

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC375  [ Marker Report ]
Locus	Xwmc375-3D Xwmc375 Xwmc375.1
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12f11 Donor: Mohler, Munich Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 197 bases located on 4A Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 173 to 188 show homology to 8h1 bases 212 to 227 (16 in longest run, maxScore = 36). (GT)9 80 to 97, (TG)7 205 to 218.
Type	PCR SSR
PCR primers	Forward cgccgAccAAAAAcTcATAA at 56 length 20 bases, Tm 63 Reverse gATTgAcATgAAAcTATggAccc at 231 length 23 bases, Tm 62
SSR size	198 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC375
Source Species	Triticum aestivum