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GrainGenes Probe Report: WMC373

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC373
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12h10.1 Donor: Mohler, Munich Trimmed to Rsa1 at both ends. Multiple inserts in this clone. This is the first insert. Bases 148 to 170 (complement) show homology to 3a12 bases 53 to 75 (23 in longest run, maxScore = 59). (CA)9 79 to 96, (CA)5 99 to 108.
Type	PCR SSR
PCR primers	Forward TAggAgTAggTcAATgTgTcgc at 24 length 22 bases, Tm 61 Reverse TATcTcAcTTccTATggcTTcg at 139 length 22 bases, Tm 60
SSR size	137 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (though complex).
Sequence	WMC373
Source Species	Triticum aestivum