Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC370

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC370
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12a10 Donor: Mohler, Munich Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 234 to 256 (complement) show homology to 5e12 bases 669 to 691 (23 in longest run, maxScore = 45). (CA)8 397 to 412, (CA)13 466 to 491, plus GCA and interspersed CA repeats. Reverse primer is shoehorned into this region.
Type	PCR SSR
PCR primers	Forward cTcTTTTgccccTcAAgTgg at 242 length 20 bases, Tm 64 Reverse gTgTgcTgTTgcgTgccTAT at 521 length 20 bases, Tm 64
SSR size	299 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear.
Sequence	WMC370
Source Species	Triticum aestivum