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GrainGenes Probe Report: WMC369

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC369
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12d9 Donor: Mohler, Munich Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 239 bases located on 1D Trimmed to insert at 5' end and to Rsa1 at 3' end. Interspersed CA microsatellite.
Type	PCR SSR
PCR primers	Forward gcAcAAcAAcAcccAAgcAAcT at 95 length 22 bases, Tm 66 Reverse TgcccTccATATcTTcTTgAcg at 311 length 65 bases, Tm 65
SSR size	238 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC369
Source Species	Triticum aestivum