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GrainGenes Probe Report: WMC368

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC368
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12a9 Donor: Mohler, Munich Trimed to Rsa1 at both ends. Bases 461 to 481 (complement) show homology to 11h2 bases 90 to 110 (21 in longest run, maxScore = 113). (CA)14 233 to 260. Forward primer has no homology to 11h2, reverse primer has 2 mismatches. Bases 372 to 404 show homology to 22g11 bases 340 to 372 (33 in longest run, maxScore = 131). Bases 404 to 447 show homology to 21a9 bases 95 to 138 (44 in longest run, maxScore = 113). Bases 32 to 100 show homology to 15A1 bases 235 to 303 (69 in longest run, maxScore = 116).
Type	PCR SSR
PCR primers	Forward TTTAgcATgTggcTTTccgTc at 163 length 21 bases, Tm 64 Reverse AgTggTcgccATATAgccTTg at 307 length 21 bases, Tm 63
SSR size	165 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear.
Sequence	WMC368
Source Species	Triticum aestivum