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GrainGenes Probe Report: WMC367

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC367  [ Marker Report ]
Locus	Xwmc367 Xwmc367-1B wmc367
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12d8.2 Donor: Mohler, Munich Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 104 bases located on 1B Trimmed to insert at 5' and and to Rsa1 at 3' end. Multiple inserts (2) in this clone. This is the second insert. Small microsatellite (GCC)5 125 to 139.
Type	PCR SSR
PCR primers	Forward cTgAcgTTgATgggccAcTATT at 8 length 22 bases, Tm 65 Reverse gTggTggAAgAggAAggAgAgg at 140 length 20 bases, Tm 65
SSR size	154 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC367
Source Species	Triticum aestivum