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GrainGenes Probe Report: WMC366

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC366  [ Marker Report ]
Locus	Xwmc366 Xwmc366-3B wmc366
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12d8.1 Donor: Mohler, Munich Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 112 bases located on 3B Trimmed to insert at both ends. Multiple inserts (2) in this clone. This is the first insert. Bases 43 to 60 (complement) show homology to 9d4 bases 379 to 396 (18 in longest run, maxScore = 28). (CA)12 121 to 144, (CA)6 174 to 185.
Type	PCR SSR
PCR primers	Forward TAccTcTcTAcgATgAAgcc at 61 length 20 bases, Tm 56 Reverse TggAgTcTTAgTgTggTgTT at 151 length 20 bases, Tm 55
SSR size	110 bases expected, Chinese Spring
Amplification Conditions	51C anneal, smear.
Sequence	WMC366
Source Species	Triticum aestivum