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GrainGenes Probe Report: WMC364

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC364  [ Marker Report ]
Locus	Xwmc364-7B Xwmc364a wmc364
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12d7 Donor: Mohler, Munich Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 188 bases located on 7D, 207 bases located on 7B Trimmed to Rsa1 at both ends. Bases 32 to 41 (complement) show homology to 13e1 bases 81 to 90 (10 in longest run, maxScore = 29). (CA)18 93 to 128.
Type	PCR SSR
PCR primers	Forward ATcAcAATgcTggcccTAAAAc at 41 length 22 bases, Tm 64 Reverse cAgTgccAAAATgTcgAAAgTc at 223 length 22 bases, Tm 64
SSR size	204 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC364
Source Species	Triticum aestivum