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GrainGenes Probe Report: WMC360

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC360  [ Marker Report ]
Locus	Xwmc360-2B Xwmc360
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 19e5 Donor: Pioneer Trimmed to insert at both ends. (GT)22 54 to 97. Software struggled to get these primers.
Type	PCR SSR
PCR primers	Forward TATgTgTgTgAAAAATgg at 2 length 18 bases, Tm 50 Reverse AATTTcTTgTAgAAccgA at 136 length 18 bases, Tm 50
SSR size	152 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.
Sequence	WMC360
Source Species	Triticum aestivum