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GrainGenes Probe Report: WMC357

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC357  [ Marker Report ]
Locus	Xwmc357-5D Xwmc357 wmc357
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 19f4 Donor: Pioneer Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 124 bases located on 5D, 137 bases located on 5D, 207 bases located on 5D Trimmed to insert at both ends. (GA)10 302 to 321, (GT)10 266 to 285.
Type	PCR SSR
PCR primers	Forward TAgTgggTgAccggTcAAgA at 159 length 20 bases, Tm 63 Reverse TggAcggATTTggTcATTTc at 343 length 20 bases, Tm 63
SSR size	204 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC357
Source Species	Triticum aestivum