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GrainGenes Probe Report: WMC356

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC356  [ Marker Report ]
Locus	Xwmc356-2B wmc356
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 19c4 Donor: Pioneer Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 245 bases located on 2A, 2B Trimmed to insert at both ends. (GT)23 146 to 191, (GT)8 206 to 221.
Type	PCR SSR
PCR primers	Forward gccgTTgcccAATgTAgAAg at 67 length 20 bases, Tm 65 Reverse ccAgAgAAAcTcgccgTgTc at 289 length 20 bases, Tm 65
SSR size	242 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (there appear to be nulls of this locus in many wheats).
Sequence	WMC356
Source Species	Triticum aestivum