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GrainGenes Probe Report: WMC355

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC355
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 19b4 Donor: Pioneer Trimmed to insert at both ends. Bases 280 to 305 show homology to 14g5.1 bases 195 to 220 (26 in longest run, maxScore = 68), zone is 118-335. Forward primer has 5 mismatches to 14g5.1, reverse primer is outside this zone of homology. (CA)14 280 to 307. Bases 216 to 228 show homology to 2b1 bases 153 to 165 (complement) (13 in longest run, maxScore = 25).
Type	PCR SSR
PCR primers	Forward gATAgTcgAAcgTTgTAggTAggT at 132 length 24 bases, Tm 59 Reverse gAAgAgAgTggAggTATcATTggT at 343 length 24 bases, Tm 61
SSR size	235 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product.
Sequence	WMC355
Source Species	Triticum aestivum