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GrainGenes Probe Report: WMC353

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC353
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 19c3 Donor: Pioneer Trimmed to insert at both ends (note Rsa1 sites at 23-27, and 27-30). Bases 27 to 64 (complement) show homology to 4d10 bases 145 to 182 (38 in longest run, maxScore = 53), zone is 27-134. Forward primer has 2 mismatches to 4d10, reverse primer is outside this region. (GT)18 106 to 141. Bases 7 to 49 show homology to 22d9 bases 10 to 52 (43 in longest run, maxScore = 54).
Type	PCR SSR
PCR primers	Forward gcTTcgcAcTcgcATgATTT at 55 length 20 bases, Tm 65 Reverse AgTAggTcgATcTgTcgcTcTc at 164 length 22 bases, Tm 62
SSR size	131 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC353
Source Species	Triticum aestivum