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GrainGenes Probe Report: WMC352

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC352
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 19h2 Donor: Pioneer Trimmed to insert at both ends. Bases 150 to 204 show homology to 9f3.1 bases 222 to 276 (55 in longest run, maxScore = 60), zone is 135-230. Reverse primer has 2 mismatches to 9f3.1, forward primer is not with the region of homology. (GA)33 80 to 145.
Type	PCR SSR
PCR primers	Forward gTTTTTgTgTcggATgcgTg at 37 length 20 bases, Tm 64 Reverse cTcccggAcTcATgTTcTcT at 141 length 20 bases, Tm 61
SSR size	124 bases expected, Chinese Spring
Sequence	WMC352
Source Species	Triticum aestivum