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GrainGenes Probe Report: WMC346

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC346  [ Marker Report ]
Locus	Xwmc346
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12g6 Donor: Weibull, Svaloff Weibull Trimmed to Rsa1 at both ends. (GA)8 299 to 314, (GT)13 7 to 32,
Type	PCR SSR
PCR primers	Forward cTgAAgTTccAgccAAcAcA at 214 length 20 bases, Tm 62 Reverse ATTcccTcATcccgTTgc at 400 length 17 bases, Tm 65
SSR size	203 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Sequence	WMC346
Source Species	Triticum aestivum