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GrainGenes Probe Report: WMC344

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC344  [ Marker Report ]
Locus	Xwmc344-2B Xwmc344 wmc344-2B wmc344
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12a6 Donor: Weibull, Svaloff Weibull Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)16 189 to 220.
Type	PCR SSR
PCR primers	Forward ATTTcAgTcTAATTAgcgTTgg at 80 length 22 bases, Tm 57 Reverse AAcAAAgAAcATAATTAAcccc at 304 length 22 bases, Tm 56
SSR size	246 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide (multi-locus polymorphic)
Sequence	WMC344
Source Species	Triticum aestivum