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GrainGenes Probe Report: WMC343

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC343  [ Marker Report ]
Locus	Xwmc343
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12h5.2 Donor: Weibull, Svaloff Weibull Multiple inserts (2) in this clone. This is the second insert. Trimmed to insert at both ends. Bases 493 to 562 show homology to 13g1.2 bases 406 to 475 (70 in longest run, maxScore = 105), zone is 381-567. Reverse primer has 6 mismatches to 13G1.2, forward primer is outside of this zone. (GT)8 326 to 341, (GT)31 346 to 407.
Type	PCR SSR
PCR primers	Forward cTTTcccAccccAcTAgATg at 262 length 20 bases, Tm 61 Reverse ccAccgTTAgTggccTATgc at 476 length 20 bases, Tm 64
SSR size	234 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC343
Source Species	Triticum aestivum