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GrainGenes Probe Report: WMC340

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC340
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 12e5
Donor: Weibull, Svaloff Weibull
Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 161 to 184 (complement) show homology to 6c1.1 bases 79 to 102 (24 in longest run, maxScore = 53), zone is 160-252. Primers do not overlap this zone. (GT)14 57 to 84, (CT)16 21 to 52.
Type
PCR
SSR
PCR primers
Forward gAggAgTTcAcTcAcccTTcT at 2 length 21 bases, Tm 59
Reverse gTTgggATcggATcgAgTA at 90 length 19 bases, Tm 61
SSR size
107 bases expected, Chinese Spring
Amplification Conditions
51C anneal, product.
Sequence
WMC340
Source Species
Triticum aestivum