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GrainGenes Probe Report: WMC339

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC339  [ Marker Report ]
Locus	Xwmc339-1D wmc339
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12b5 Donor: Weibull, Svaloff Weibull Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 208 bases located on 6B Trimmed to Rsa1 at 5' end and to insert at 3' end. (GA)8 118 to 133.
Type	PCR SSR
PCR primers	Forward ccgcTcgccTTcTTccAg at 29 length 18 bases, Tm 66 Reverse TccggAAcATgccgATAc at 215 length 18 bases, Tm 64
SSR size	204 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC339
Source Species	Triticum aestivum