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GrainGenes Probe Report: WMC337

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC337
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12d4 Donor: Weibull, Svaloff Weibull Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 80 to 134 show homology to 1B4 bases 156 to 210 (55 in longest run, maxScore = 51), zone 79-241 Reverse primer has 4 mismatches to 1B4 sequence. (GT)33 65 to 130.
Type	PCR SSR
PCR primers	Forward cgTcTAggATTTTAcAAAccAc at 43 length 22 bases, Tm 57 Reverse gAcTTAAcccATTTAgcTTcAT at 133 length 22 bases, Tm 56
SSR size	112 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC337
Source Species	Triticum aestivum