Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC334

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC334  [ Marker Report ]
Locus	Xwmc334 wmc334
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12b3 Donor: Weibull, Svaloff Weibull Trimmed to insert at 5' end and to Rsa1 at 5' end. (CA)5G(CA)6 at 162 to 185.
Type	PCR SSR
PCR primers	Forward cTcgAAgcAggATgcATTTA at 89 length 20 bases, Tm 61 Reverse TcAAATTTTgggATAgcggg at 186 length 20 bases, Tm 64
SSR size	117 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC334
Source Species	Triticum aestivum