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GrainGenes Probe Report: WMC331

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC331  [ Marker Report ]
Locus	Xwmc331-4D Xwmc331 Alt2 wmc331
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 17f11 Donor: Khairallah, Mexico Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 134 bases located on 4D Trimmed to insert at 5' end and to insert at 3' end. (CA)13 56 to 81, (CT)10 36 to 55. Software struggled to get these primers.
Type	PCR SSR
PCR primers	Forward ccTgTTgcATAcTTgAccTTTTT at 2 length 23 bases, Tm 61 Reverse ggAgTTcAATcTTTcATcAccAT at 107 length 23 bases, Tm 61
SSR size	128 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide.
Sequence	WMC331
Source Species	Triticum aestivum