Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC330

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC330
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 17a11 Donor: Khairallah, Mexico Trimmed to insert at both ends (NB 3' linker corrupted). Bases 1 to 34 show homology to 4a10 bases 98 to 131 (34 in longest run, maxScore = 32), zone is 1-72. Forward primer has 1 mismatch to 4a10, reverse primer is outside this region. (GA)15 46 to 75, (GA)13 88 to 113.
Type	PCR SSR
PCR primers	Forward gcgTgcgAcAgAAcTAAAgA at 20 length 20 bases, Tm 61 Reverse cAcAAAccTTgTTggTcccT at 135 length 20 bases, Tm 62
SSR size	135 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear; 51C anneal, smear.
Sequence	WMC330
Source Species	Triticum aestivum