Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC328

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC328
Locus	Xwmc328
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 17f9 Donor: Khairallah, Mexico Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 15 to 83 (complement) show homology to 6d3 bases 243 to 311 (69 in longest run, maxScore = 58), zone is 1-83. Bases 23 to 50 show homology to 19g2 bases 70 to 97 (complement) (28 in longest run, maxScore = 45). Forward primer has mismatch to 6d3 and 19g2. (CT)10 103 to 122. Bases 50 to 101 show homology to 26h4 bases 323 to 374 (complement) (52 in longest run, maxScore = 79).
Type	PCR SSR
PCR primers	Forward ATTgcAgTTcTAcccTcAgT at 4 length 20 bases, Tm 56 Reverse gggTcgAcATccATAcATAA at 149 length 20 bases, Tm 58
SSR size	165 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC328
Source Species	Triticum aestivum