Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC327

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC327  [ Marker Report ]
Locus	Xwmc327-5A Xwmc327 wmc327 wmc327-5A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 17e9 Donor: Khairallah, Mexico Trimmed to insert at both ends. Bases 1 to 15 show homology to 1H3 bases 116 to 130 (15 in longest run, maxScore = 24), zone is 1-40. Primers are outside of this region. (GT)25 150 to 199.
Type	PCR SSR
PCR primers	Forward TgcggTAcAggcAAggcT at 75 length 18 bases, Tm 65 Reverse TAgAAcgcccTcgTcggA at 240 length 18 bases, Tm 65
SSR size	183 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Sequence	WMC327
Source Species	Triticum aestivum