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GrainGenes Probe Report: WMC325

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC325
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 17h7 Donor: Khairallah, Mexico Trimmed to insert at both ends. Bases 417 to 457 (complement) show homology to 13c5 bases 249 to 289 (41 in longest run, maxScore = 82) zone is 227-488, primers apparently ouside this homology. (CA)15 60 to 89, (CT)13 34 to 59. Bases 131 to 151 show homology to 24d6 bases 144 to 164 (21 in longest run, maxScore = 38). Bases 245 to 307 show homology to 15a4 bases 301 to 363 (63 in longest run, maxScore = 157).
Type	PCR SSR
PCR primers	Forward gcAgAgcAccTTTTgTTTTgAg at 4 length 22 bases, Tm 63 Reverse gccggAAAAAATgAAgcgAgAA at 94 length 22 bases, Tm 68
SSR size	112 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC325
Source Species	Triticum aestivum