Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC322

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC322
Locus	Xwmc322-3A Xwmc322 Xwmc322-3B wmc322 wmc322-3A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 17b7 Donor: Khairallah, Mexico Trimmed to insert at both ends. (GT)24 40 to 87. Forward primer reduced size to lower Tm.
Type	PCR SSR
PCR primers	Forward cgccccAcTATgcTTTg at 12 length 17 bases, Tm 61 Reverse cccAgTccAgcTAgccTcc at 88 length 19 bases, Tm 64
SSR size	95 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide.
Sequence	WMC322
Source Species	Triticum aestivum