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GrainGenes Probe Report: WMC321

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC321
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14e6 Donor: Hjortsholm, Sejet Trimmed to insert at both ends. Bases 57-191 complement match part of 13H3. Bases 148 to 180 (complement) show homology to 13h3 bases 13 to 45 (33 in longest run, maxScore = 62). (GA)9 286 to 303, (GA)8 314 to 329.
Type	PCR SSR
PCR primers	Forward gggAgcAgAcTATTTggAAgAg at 230 length 22 bases, Tm 61 Reverse cgccATTTATggAcAcAAA at 357 length 19 bases, Tm 60
SSR size	146 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC321
Source Species	Triticum aestivum