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GrainGenes Probe Report: WMC319

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC319
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14g5.1 Donor: Hjortsholm, Sejet Trimmed to insert at both ends. Multiple inserts in this clone - this is first insert. Bases 195 to 220 show homology to 19b4 bases 280 to 305 (26 in longest run, maxScore = 68). Forward primer has 4 mismatches to 19B4, reverse primer is outside this region (CA)15 191 to 220.
Type	PCR SSR
PCR primers	Forward cgAgcgcTAcAggTAggTATcc at 53 length 22 bases, Tm 64 Reverse gAggTATcATcggcTTgAggTg at 246 length 22 bases, Tm 65
SSR size	215 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC319
Source Species	Triticum aestivum