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GrainGenes Probe Report: WMC318

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC318  [ Marker Report ]
Locus	Xwmc318 Xwmc318-5D wmc318
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14e5 Donor: Hjortsholm, Sejet Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 207 bases located on 5D Trimmed to insert at 5' ends and to Rsa1 at 3' end. Bases 57-149 match part of 1g5. Bases 84 to 97 show homology to 1G5 bases 89 to 102 (14 in longest run, maxScore = 27). Forward primer does not overlap this homology. (GT)11 115 to 136. Bases 80 to 93 show homology to 10d12 bases 359 to 372 (complement) (14 in longest run, maxScore = 28).
Type	PCR SSR
PCR primers	Forward cgTAAAATTAcggTgcATTgAT at 19 length 22 bases, Tm 60 Reverse gTggAcTTTTgTggTTTTTgAg at 199 length 22 bases, Tm 60
SSR size	202 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC318
Source Species	Triticum aestivum