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GrainGenes Probe Report: WMC315

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC315
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14h2 Donor: Hjortsholm, Sejet Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 1-83 (complement) (ie 5' side of microsatellite) match part of 5g10 (primers do not come from this area). (GT)23 107 to 152, (GT)8 165 to 180, (GT)8 187 to 202,
Type	PCR SSR
PCR primers	Forward TAgTcgTTTccgcATAAAccAA at 85 length 22 bases, Tm 63 Reverse TgAggTTcAcccTATATgccAA at 247 length 22 bases, Tm 63
SSR size	184 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Sequence	WMC315
Source Species	Triticum aestivum