Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC314

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC314  [ Marker Report ]
Locus	Xwmc314
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14g2 Donor: Hjortsholm, Sejet Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 84-111 match part of 7G4 - primers do not include this zone. (GT)15 281 to 310, (GT)9 360 to 377. Bases 359 to 379 show homology to 26b2 bases 109 to 129 (21 in longest run, maxScore = 23).
Type	PCR SSR
PCR primers	Forward AcAcggggTcTgATTgcTTTAc at 245 length 22 bases, Tm 64 Reverse ATcgcTTTTTgAcAAgTgAggc at 411 length 22 bases, Tm 64
SSR size	188 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC314
Source Species	Triticum aestivum