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GrainGenes Probe Report: WMC308

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC308
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8f6 Donor: Rouse, Novartis Contains (TG)14. Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)14 181 to 208.
Type	PCR SSR
PCR primers	Forward gAgcAgAggAAAATTcggATgA at 34 length 22 bases, Tm 64 Reverse AAATgTTgTgAAAAAgcgAgcA at 223 length 22 bases, Tm 64
SSR size	211 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide;
Sequence	WMC308
Source Species	Triticum aestivum