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GrainGenes Probe Report: WMC306

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC306
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8a5 Donor: Rouse, Novartis contains (TC)12 and (CA)22 repeats. Trimmed to rsa1 at 5' end and to insert at 3' end. Bases 1 to 86 show homology to 14c5 bases 1 to 86 (86 in longest run, maxScore = 62). (CA)22 88 to 131, (CT)11 62 to 83. Forward primer is within homology to 14c5.
Type	PCR SSR
PCR primers	Forward TTgcTTccTTgcTcgATgc at 27 length 19 bases, Tm 64 Reverse ccAAcgAATgAgccgATgT at 146 length 19 bases, Tm 64
SSR size	138 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product. Not polymorphic on tested varieties.
Sequence	WMC306
Source Species	Triticum aestivum