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GrainGenes Probe Report: WMC304

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC304  [ Marker Report ]
Locus	Xwmc304 wmc304 wmc304-1A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8g4 Donor: Rouse, Novartis Trimmed to insert at 5' end and to Rsa1 at 3' end. (CT)20 179 to 218. Bases 3-65 match part of 6b10 - primers outside of this area. Bases 177 to 218 show homology to 22a7 bases 193 to 234 (42 in longest run, maxScore = 58), true duplicate, 8g4 has priority. Bases 173 to 218 show homology to 25c7 bases 100 to 145 (complement) (46 in longest run, maxScore = 106).
Type	PCR SSR
PCR primers	Forward cgATAcAAggAAgAccAgcc at 100 length 20 bases, Tm 61 Reverse ggTTcgTcTggTTcgcAAgT at 219 length 20 bases, Tm 64
SSR size	139 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Sequence	WMC304
Source Species	Triticum aestivum