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GrainGenes Probe Report: WMC303

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC303
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8d4 Donor: Rouse, Novartis Contains (AG)22. Trimmed to Rsa1 at both ends. (GA)21 292 to 333. Bases 12-39 show homology to 3b2 (primers outside this region).
Type	PCR SSR
PCR primers	Forward cAAAATgTcccAgccAAAgAgT at 258 length 22 bases, Tm 64 Reverse gcTcTTgAgcTggTgTgATgAA at 357 length 22 bases, Tm 65
SSR size	121 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, complex products.
Sequence	WMC303
Source Species	Triticum aestivum