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GrainGenes Probe Report: WMC297

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC297
Locus	wmc297-6A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 13g5 Donor: Jager-Gussen, Saaten Union Trimmed to insert at 5' end and to Rsa1 at 3' end. (GT)29 188 to 245. Bases 117 to 245 show homology to 19h3 bases 14 to 142 (complement) (129 in longest run, maxScore = 57).
Type	PCR SSR
PCR primers	Forward TcAAAAgccTcAAAgAAAcTcA at 59 length 22 bases, Tm 61 Reverse GATAATAAgAATcccATggAAAcA at 257 length 24 bases, Tm 60
SSR size	220 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Sequence	WMC297
Source Species	Triticum aestivum