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GrainGenes Probe Report: WMC296

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC296  [ Marker Report ]
Locus	Xwmc296-2A [ Show all 8 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 13d5.1 Donor: Jager-Gussen, Saaten Union Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 165 bases located on 2A Trimmed to insert at both ends. Multiple inserts (2) joined 377- 394 by linkers - this is first insert. (GA)11 233 to 254, (GT)28 177 to 232.
Type	PCR SSR
PCR primers	Forward gAATcTcATcTTcccTTgccAc at 127 length 22 bases, Tm 64 Reverse ATggAggggTATAAAgAcAgcg at 260 length 22 bases, Tm 63
SSR size	155 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Sequence	WMC296
Source Species	Triticum aestivum