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GrainGenes Probe Report: WMC295

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC295
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 13b4 Donor: Jager-Gussen, Saaten Union Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)8 144 to 159, (CA)9 162 to 179.
Type	PCR SSR
PCR primers	Forward ccATAAgTgTTTgcgTTTATTcc at 73 length 23 bases, Tm 61 Reverse AATgcAcTATTTTTATAgcTTTgT at 187 length 24 bases, Tm 56
SSR size	138 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product.
Sequence	WMC295
Source Species	Triticum aestivum