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GrainGenes Probe Report: WMC294

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC294
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 13a4 Donor: Jager-Gussen, Saaten Union Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 230 bases located on 3D Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 1-158 (complement) (ie. 5' side of microsatellite) match part of 7A3(3) - forward primer has 4 mismatches to 7A3(3) including 3' base.. Complex microsatellite (TC)8(CA)7(GCA)2(CA)2(GCA)(CA).
Type	PCR SSR
PCR primers	Forward ccgccTgTgTgAAATTTTTgTT at 8 length 22 bases, Tm 65 Reverse TTAAAAgcTgTcTcATgcgAAg at 213 length 22 bases, Tm 61
SSR size	227 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide.
Sequence	WMC294
Source Species	Triticum aestivum