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GrainGenes Probe Report: WMC293

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC293
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 13d3 Donor: Jager-Gussen, Saaten Union Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 131 bases located on 7A, 170 bases located on 7A Trimmed to insert at both ends. Short (CGG)6 microsatellite 101-119. Weak homology to 11g4.
Type	PCR SSR
PCR primers	Forward cgTcTcTTTcATgTcAcTgccc at 63 length 22 bases, Tm 66 Reverse AgTTTcgTcgcggTTcTcTggA at 171 length 22 bases, Tm 69
SSR size	130 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC293
Source Species	Triticum aestivum