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GrainGenes Probe Report: WMC291

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC291  [ Marker Report ]
Locus	Xwmc291 Xwmc291-3B wmc291
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 13f2 Donor: Jager-Gussen, Saaten Union Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 239 bases located on 3B Trimmed to insert at 5' end and to insert at 3' end. (GT)26 174 to 225.
Type	PCR SSR
PCR primers	Forward TAccAcgggAAAggAAAcATcT at 26 length 22 bases, Tm 63 Reverse cAcgTTgAAAcAcggTgAcTAT at 237 length 22 bases, Tm 62
SSR size	233 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC291
Source Species	Triticum aestivum